Institut für Radiologie und Kinderradiologie

Charité – Universitätsmedizin Berlin

Campus Mitte

Charitéplatz 1, 10117 Berlin

Telephone: +49 30 450 539 074

Email: azadeh.mohtashamdolatshahi@charite.de

 

Towards quantitative structure-sensitive MPI: Application for sentinel lymph node detection

PIs: Schnorr, Taupitz, Schäffter, Käs

Application area: Cancer

Modalities: MPI, MRI

Background: The novel Magnetic Particle Imaging (MPI) modality detects and localizes magnetic nanoparticles (MNP) with high sensitivity. MPI has the potential to quantitatify MNP and their interaction with tissue environments, such as adhesion of MNP to macromolecular, ECM-, and cell surface components as well as internalization by phagocytosing cells. Experimental interstitial lymphography (IL) with sentinel lymph node targeting offers an ideal model to study interactions of MNP with different environments on a slow time scale and to study how these interactions influence the MPI signal.

Aim: Quantification of MNPs and their interactions with various environments in models of IL.

Methods: MNPs in various media and environments such as (in vitro models) water, blood, serum, lymph fluid, gel assays, cell cultures, (ex vivo models) lymph nodes and (in vivo models) injection site, lymph vessels, lymph nodes. Correlation with MPI, Magnetic Particle Spectroscopy (MPS), magnetorelaxometry, MRI, light scattering, iron analytics, histology, transmission electron microscopy (TEM).